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blat

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36 (with

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options)

Blat_with_options is an app for Blat, the Blast-Like Alignment Tool. This version allows setting options.

Quick Start

Test Data

Info

Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> Blat

Input File(s)

Use the E. coli K12 genome from http://www.ncbi.nlm.nih.gov/nuccore/NC_010473.1?report=fasta&log$=seqview&format=text
and the homoserine kinase gene sequence from http://www.ncbi.nlm.nih.gov/nuccore/NC_010473.1?report=fasta&log$=seqview&format=text&from=2801&to=3733 as test data. Use Import from URL to get the data into your Discovery Environment account.

Arabidopsis_thaliana.TAIR10.fa as the database file and

AT3G24310.fa as the query file

Parameters Used in App

There following settings can be used:

...

Expect a tab delimited txt file as output, named output.psl or whatever you changed it to. It is in psl format, which can be converted to SAM format.

Tool Source for App