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NCBI fastq-dump can be very slow sometimes, even if you have the resources (network, IO, CPU) to go faster, even if you already downloaded the sra file (see the protip below). This tool speeds up the process by dividing the work into multiple threads. This is possible because fastq-dump have options (-N and -X) to query specific ranges of the sra file, this tool works by dividing the work into the requested number of threads, running multiple fastq-dump in parallel and concatenating the results back together, as if you had just executed a plain fastq-dump call.

Image Modified

parallel-fastq-dump-multi-0.6.5 is invoked using the following:

  1. Input (s)
    1. File containing SRA ids (1 SRA id per line)
  2. Parameters
  3. Outputs
    1.  Output Folder Name (default - sra_out)

Please work through the documentation and add your comments on the bottom of this page, or email comments to Thank you.

Test Data

All files are located in the Community Data directory of the CyVerse Discovery Environment at the following path:

Community Data > iplantcollaborative > example_data > ncbi_sra_toolkit_fastq_dump (/iplant/home/shared/iplantcollaborative/example_data/ncbi_sra_toolkit_fastq_dump)

 Run parallel-fastq-dump-multi-0.6.5 as following:

  1. Input file
    1. sra_id_se.txt
  2. Parameters
  3. Outputs
    1. Output Folder Name (default - sra_out)

Tool Source for App