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- To use Gfold 1.1.1 Count, please have the following files available:
- A gene/genome annotation file in these formats: *.GTF ; *.GPF ; or *.BED
- A Sequence Alignment/Map file in: SAM format
- If you have BAM (binary version of SAM file) from Tophat2 - Single End or Tophat2 - Paired End : please use SAMTOOLS-0.1.19 BAM-to-SAM to convert the file into SAM format
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Community Data -> iplant -> home -> shared -> iplant_training -> intro_rna-seq -> 02_tophat -> bam
(Please run SAMTOOLS-0.1.19 BAM-to-SAM on the following files)
2a) *Input file: WT_rep1.bam
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