Polymarker
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PolyMarker is an automated bioinformatics pipeline for SNP assay development which increases the probability of generating homoeologue-specific assays for polyploid organisms. PolyMarker generates a multiple alignment between the target SNP sequence and the chromosome survey sequences. It then generates a mask with informative positions which are highlighted with respect to the target genome. Because many users are analysing the wheat genome this is provided and doesn't need to be uploaded.
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Test data for this app are temporarily not available in the Discovery Environment under >Shared with me>rosysnake>example_polymarker. |
Input File(s)
The input file must be uploaded as a CSV file with the following columns:
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There are no required parameters, the app can be run with the default settings. For a list of possible options see the link at the bottom of the page.
Output File(s)
The mask contains the details of the local alignment. Multiple intermediate files will also be available.
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