Genome file requirements depend on the file type. Bam and vcf files require a matching index file (bam.bai or vcf.vci) to tell it which part of the genome to load, and both files must in the same directory with the same name. For example, if the file is named sample.bam, its index file must be in the same directory and named sample.bam.bai.
Gff, gtf, bed, bigBed, and bigWig files require that the name of the reference genome's fasta header match the gene name in the gff, gtf, bed, bigBed and bigWig file. |