BWA Aligner for Paired-End Illumina Reads
App Name
BWA Burrows-Wheeler Aligner (BWA) is an efficient program that aligns relatively short nucleotide sequences against a long reference sequence such as the human genome. This instance of BWA works with paired-end sequencing reads.
Quick Start
- To use BWA, upload your data in FASTQ format.
- Resources: http://bio-bwa.sourceforge.net/bwa.shtml
Test Data
Input File(s)
Use 10K_SRR192294_1.fastq (2.4M) http://mirrors.iplantcollaborative.org/example_data/bwa/10K_SRR192294_1.fastq and 10K_SRR192294_2.fastq (2.4M) http://mirrors.iplantcollaborative.org/example_data/bwa/10K_SRR192294_2.fastq as test data. Import from URL to get the data into your Discovery Environment account.
Parameters Used in App
When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.
- Select a reference genome from the list - Brachypodium distachyon (line Bd21) v1
- All other parameters remain in their default state
Output File(s)
Expect a SAM file named after the input files as output. For the test case, the output file is 10K_SRR192294_1.fastq-10K_SRR192294_2.fastq.sam (4.8M) http://mirrors.iplantcollaborative.org/example_data/bwa/10K_SRR192294_1.fastq-10K_SRR192294_2.fastq.sam