gbsAlign_BWA 0.5.9
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gbsAlign_BWA 0.5.9Â
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BWAÂ Burrows-Wheeler Aligner (BWA) is an efficient program that aligns relatively short nucleotide sequences against a long reference sequence such as the human genome. This instance of BWA works with single-end sequencing reads.
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Quick Start
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- To use BWA, upload your data in FASTQ format.
- Resources:Â http://bio-bwa.sourceforge.net/bwa.shtml
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Test Data
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Input File(s)
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Use 10K_SRR192294_1.fastq (2.4M) http://mirrors.iplantcollaborative.org/example_data/bwa/10K_SRR192294_1.fastq as test data. Import from URL to get the data into your Discovery Environment account.
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Parameters Used in App
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When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.
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- Select a reference genome from the list - Brachypodium distachyon (line Bd21) v1
- All other parameters remain in their default state
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Output File(s)
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Expect a SAM file named after the input file as output. For the test case, the output file is 10K_SRR192294_1.fastq.sam (2.2M)http://mirrors.iplantcollaborative.org/example_data/bwa/10K_SRR192294_1.fastq.sam
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Tool Source for App
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