2014 05 20 iPlant Data Clinic Reno

2014 05 20 iPlant Data Clinic Reno

Organizers

Roger Barthelson, Christie Howard (UNVReno)

iPlant Trainers

Roger Barthelson & Uwe Hilgert

Date

May 20, 2014

Location

UNV Reno
Mathewson-IGT Knowledge Center;
Computer Training Lab, room number 114, first floor.
Look for the stairwell next to the main entrance to take you downstairs to the @One computer labs.
Room 114 is a private room in the back of the @One center. For questions ask the information desks at the second floor entrance or in @One.

Parking

Brian Whalen parking garage complex on N. Virginia Street. (Right side of North Virginia street just before the Lawlor events center.) 
Park on top floor and obtain a permit at the kiosk on the top floor of the Whalen Parking complex. (Use code '#' '8889999' for free parking; press the # sign and then 8889999.)
Place ticket in your car window and take the elevator or stairs next to the kiosk to ground level.
The Mathewson-IGT Knowledge Center will be on campus to the left as you exit the garage.

Designated Lodging

Holiday Inn Express, 2375 Market Street, Reno Nevada  89502

Workshop Checklist

1. iPlant Account

2. Atmosphere Access

3. Uploaded Data (Optional)

If you are bringing data to the workshop, please follow the instructions below. It is recommended to upload your data before attending the workshop. You should upload from the best possible internet connection, and if you have any issues email support@iplantcollaborative.

Depending on your level of comfort and the size of the data, you may want to try one of two methods.

1)   iDrop – a desktop drag/drop utility

2)   iCommands – command line interface 

For iDrop, you can download the utility here: bit.ly/1i7wm4s

For iCommands, you can download the scripts here: bit.ly/1kNdDyR

When uploading via iCommands (using iput) it is recommended that you use the T option to renew the connection socket, especially for single large (>30GB) files. The documentation also discusses the options to display progress and to create a restart file. 

Once you have uploaded the data, please fill out this survey (https://docs.google.com/forms/d/1DdQMjk_ri9L4eEma5AOLiRsGMOPRwOB0mVezThF_W2o/viewform ) so that we can have a clearer idea of what data you will be bringing.

4. Laptop (Optional)

You may wish to bring your own WiFi enabled laptop to the workshop. We will be working from the computer lab, but you may choose to use your own laptop either during the course, or for homework. Please be sure your laptop has the following:

5. Important notes to prepare for the workshop: Pre-WorkshopHomework.pdf

Agenda

The agenda is an outline of the activities we will present at the clinic. We will make every effort to make adjustments as needed based upon the needs and interests of the participants.

Time

Activity

Instructor

Slides

Links, Notes, etc.

9 am

Intro to iPlant and a review of iPlant resources

Uwe Hilgert

iPlant Intro and Resource Review

 

10:30 am

Tutorial:Tuxedo Suite for RNA-Seq analysis

Roger Barthelson
Uwe Hilgert assisting

Introductory_RNA-seq.ppt
AltRNASeq_Annotation.pptx

denovo_transcriptome_assembly_tutorial.docx
RNASeq_wo_reference_genome_121613.docx

11:15 am

Start Tophat alignment

Roger Barthelson
Uwe Hilgert assisting

 

 

11:45 am

Discussion of Tophat output and Cufflinks

 

 

 

12:00 am

Lunch

 

 

 

1:00 am

Continue RNA-Seq Tutorial
Cufflinks
Cuffmerge
CuffDiff
CummerBund

Roger Barthelson
Uwe Hilgert assisting