2014 05 20 iPlant Data Clinic Reno
Organizers |
Roger Barthelson, Christie Howard (UNVReno) |
---|---|
iPlant Trainers |
Roger Barthelson & Uwe Hilgert |
Date |
May 20, 2014 |
Location |
UNV Reno |
Parking |
Brian Whalen parking garage complex on N. Virginia Street. (Right side of North Virginia street just before the Lawlor events center.) |
Designated Lodging |
Holiday Inn Express, 2375 Market Street, Reno Nevada 89502 |
Workshop Checklist
1. iPlant Account
- Get your free account at http://user.iplantcollaborative.org.
2. Atmosphere Access
- Once you have your account, go to https://user.iplantcollaborative.org/dashboard/ and under Available Services, request access to Atmosphere. Indicate that you are attending a workshop when you are asked for justification.
3. Uploaded Data (Optional)
If you are bringing data to the workshop, please follow the instructions below. It is recommended to upload your data before attending the workshop. You should upload from the best possible internet connection, and if you have any issues email support@iplantcollaborative.
Depending on your level of comfort and the size of the data, you may want to try one of two methods.
1) iDrop – a desktop drag/drop utility
2) iCommands – command line interface
For iDrop, you can download the utility here: bit.ly/1i7wm4s
- You can read documentation here: bit.ly/PuWXPP
- You can see a video explanation here: http://youtu.be/0YiMG3OMbhM
For iCommands, you can download the scripts here: bit.ly/1kNdDyR
- You can read documentation here: bit.ly/1frB8W1
- You can see a video explanation here: http://youtu.be/C8tLzBdNG8Y
When uploading via iCommands (using iput) it is recommended that you use the T option to renew the connection socket, especially for single large (>30GB) files. The documentation also discusses the options to display progress and to create a restart file.
Once you have uploaded the data, please fill out this survey (https://docs.google.com/forms/d/1DdQMjk_ri9L4eEma5AOLiRsGMOPRwOB0mVezThF_W2o/viewform ) so that we can have a clearer idea of what data you will be bringing.
4. Laptop (Optional)
You may wish to bring your own WiFi enabled laptop to the workshop. We will be working from the computer lab, but you may choose to use your own laptop either during the course, or for homework. Please be sure your laptop has the following:
- VNC Viewer: Download the DMG (for MAC) or exe (for PC): http://www.realvnc.com/download/viewer/
- Java: Please have JAVA installed and enabled (help)
- Browser: Please have an up-to-date web browser (Recommended Firefox or Safari )
5. Important notes to prepare for the workshop: Pre-WorkshopHomework.pdf
Agenda
The agenda is an outline of the activities we will present at the clinic. We will make every effort to make adjustments as needed based upon the needs and interests of the participants.
Time |
Activity |
Instructor |
Slides |
Links, Notes, etc. |
---|---|---|---|---|
9 am |
Intro to iPlant and a review of iPlant resources |
Uwe Hilgert |
|
|
10:30 am |
Tutorial:Tuxedo Suite for RNA-Seq analysis |
Roger Barthelson |
denovo_transcriptome_assembly_tutorial.docx |
|
11:15 am |
Start Tophat alignment |
Roger Barthelson |
|
|
11:45 am |
Discussion of Tophat output and Cufflinks |
|
|
|
12:00 am |
Lunch |
|
|
|
1:00 am |
Continue RNA-Seq Tutorial |
Roger Barthelson |
|
|