2012 08 22 Genomics in Education Workshop, UWisconsin-Madison

2012 08 22 Genomics in Education Workshop, UWisconsin-Madison

Organizers:

Jason Williams and Sheldon McKay

Local Point of Contact:

Scott Woody

Trainers:

Jason Williams, Brent Buckner

Date:

August 22-23, 2012

Location:

Computer Sciences Teaching Lab, Rm. 3280B
Wisconsin Inst. for Discovery
330 N Orchard St
University of Wisconsin at Madison, WARF

Flyer:

Section Navigation

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).

  • Ensure that your computer is running a current browser (Mozilla Firefox 13/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).

  • Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get Java 6 @ this link: http://java.com/en/download/manual_v6.jsp

  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.

  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).

  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • Review the  (a printed copy and some other handouts will be presented to you at the workshop).

  • Review the

  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.

  • If you wish to bring sequence data for the DNA Barcoding or genome annotation experiments feel free to do so! 

  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Wiki Short URL: http://www.iplantcollaborative.org/madwiki

Wedesday, August 22nd 

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

Overview, Workshop Objectives, DNA Subway Tour

 

10:15-10:30

iPlant Collaborative Overview

www.iplantcollaborative.org

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to teach concepts in Molecular Biology

,

11:15-12:00

Setting up projects for DNA annotation - Red Line

 

 

12:00-01:00

Lunch (delivered)

 

 

01:00-01:15

DNA Barcoding wet-lab setup

 

01:15-01:30

Mini-Seminar: DNA Barcoding

 

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:45

Gene Annotation I

 

 

04:45-05:00

Mini-Seminar: Alternative Splicing                                                      

, ,

Thursday, August 23rd

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              

Transposon Presentation

, , Epigenetic TE regulation in plants

09:30-10:15

Transposon Sleuthing: Yellow Line

 

 

10:15-10:30

Break

 

 

10:30-11:30

Gene Annotation II

 

 

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch (delivered)

 

 

01:30-02:30

DNA Barcoding analysis (Blue Line)

                 

 

02:30-02:45

Break

 

 

02:45-03:45

DNA Barcoding electrophoresis and sequencing prep (GENEWIZ) 

 

 

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

http://www.learnaboutsma.org/   - SMA website and splicing animation.

http://www.iBoL.org - International Barcode of Life Project

DNA Subway exercises and handouts in and format. 

DNA Barcoding

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

https://www.surveymonkey.com/s/GenomicsInEducation_post

Post Workshop DNA Sequencing Results

1.       Log into DNA Subway (dnasubway.iPlantcollaborative.org)

2.       Start a blue line project

3.       Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding

4.       Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number: 10-204444324 on 8/29/12, (Its the second entry by Jermel Watkins)

5.       Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.

           For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....

Important Pre-Workshop Travel Info

Nearest Airports/ Rail

Airport

Distance

Notes

Shuttle Services from Airports and Buses

Call ahead of time to reserve/confirm

Service

Notes

Parking Information:

On-Campus Directions:

See the Campus Map.