2012 06 29 - Genomics in Education Workshop, NDSU

2012 06 29 - Genomics in Education Workshop, NDSU

Organizers:

Shahryar F. Kianian, Jason Williams

Local Point of Contact:

Shahryar F. Kianian

Trainers:

David Micklos, Jason Williams

Date:

12:00PM - 6:00PM on June 29, 9:00AM - 5:00PM on June 30, 2012

Location:

Lecture: 380 Loftsgard hall
Lab: 210/212 Loftsgard hall

Section Navigation

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 3.6/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install the newest version.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • ReviewDNA Subway manual (a printed copy will be presented at the workshop)
  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Friday, June 29th

Time

Topic

Slides

Notes/Links

12:00-12:30

Lunch - Welcome/Logistics

 

 

12:30-12:45

Introductions

 

 

12:45-01:30

iPlant Collaborative, Workshop Objectives, DNA Subway Tour

Slides

 

01:30-02:00

Using DNA Subway to Teach Concepts in Molecular Biology

Slides

 

02:00-02:15

Break

 

 

02:15-03:00

Setting up projects for Annotation - Red Line

 

 

03:00-03:15

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:00

Gene Annotation I

 

 

04:00-04:15

Break

 

 

04:15-04:30

Mini-Seminar: DNA Barcoding

Slides

 

04:30-05:45

DNA Barcoding - DNA Isolation

 

 

Saturday, June 30th

Time

Topic

Slides

Notes/Links

09:00-09:30

DNA Barcoding - PCR                      


 

09:30-10:00

Mini-seminar: Alternative Splicing

Animations

 

10:00-10:30

Gene Annotation II

 

 

10:30-10:45

Break

 

 

10:45-11:15

Gene Annotation II

 

 

11:15-11:45

Mini-Seminar: Transposons

Slides

 

11:45-12:30

Prospecting Transposons in Genomes - Yellow Line

 

 

12:30-01:30

Lunch

 

 

01:30-02:30

DNA Barcdoing electrophoresis and sequencing prep

Gel Photo

 

02:30-03:30

DNA Barcoding - Blue Line

 

 

03:30-03:45

Break

 

 

03:45-04:30

Planning and Classroom Implementation

 

 

04:30-05:00

Evaluation and Graduation

 

 

Other Websites and Links and Resources

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

Post Workshop DNA Sequencing Results

Instructions on retrieving your results will be posted here after the workshop.

Important Pre-Workshop Travel Info

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Check website like hotels.com and travelocity.com for additional hotels and rates. 

Nearest Airports/ Rail

Airport

Distance

Notes

Fargo Hector International Airport

~3 Miles


Taxi rates and rentals info:

Call ahead of time to reserve/confirm

Service

Notes

http://www.fargoairport.com/car-rental.html

From airport website

Parking Information:

Main campus there is a visitors parking lot right in front of Loftsgard hall.  Summer and very flexible, so people can park almost anywhere.  It is a weekend and campus police does not ticket people on weekends.

On-Campus Directions:

http://www.ndsu.edu/ndsu/main/directory/bldg_list.php