2012 06 29 - Genomics in Education Workshop, NDSU
Organizers: |
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Local Point of Contact: |
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Trainers: |
David Micklos, Jason Williams |
Date: |
12:00PM - 6:00PM on June 29, 9:00AM - 5:00PM on June 30, 2012 |
Location: |
Lecture: 380 Loftsgard hall |
Section Navigation
Pre-Workshop Homework
Laptop Compatibility
If you are bringing your own laptop:
Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.
Here are some things to check:
- If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
- Ensure that your computer is running a current browser (Mozilla Firefox 3.6/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
- If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install the newest version.
- If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
- To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot
Make sure you have a valid iPlant account
- You should be able to log into the DNA subway at: http://dnasubway.iplantcollaborative.org/.
- If you do not have an iPlant account, please get one at: http://user.iplantcollaborative.org/register.
Additional Readings and Assignments
- ReviewDNA Subway manual (a printed copy will be presented at the workshop)
- Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
- Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
- If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so!
- Check out the slides and papers mentioned in the agenda.
Workshop Agenda and Resources
Friday, June 29th
Time |
Topic |
Slides |
Notes/Links |
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12:00-12:30 |
Lunch - Welcome/Logistics |
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12:30-12:45 |
Introductions |
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12:45-01:30 |
iPlant Collaborative, Workshop Objectives, DNA Subway Tour |
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01:30-02:00 |
Using DNA Subway to Teach Concepts in Molecular Biology |
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02:00-02:15 |
Break |
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02:15-03:00 |
Setting up projects for Annotation - Red Line |
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03:00-03:15 |
Mini-Seminar: Evidence Based Gene Modeling |
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03:15-04:00 |
Gene Annotation I |
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04:00-04:15 |
Break |
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04:15-04:30 |
Mini-Seminar: DNA Barcoding |
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04:30-05:45 |
DNA Barcoding - DNA Isolation |
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Saturday, June 30th
Time |
Topic |
Slides |
Notes/Links |
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09:00-09:30 |
DNA Barcoding - PCR |
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09:30-10:00 |
Mini-seminar: Alternative Splicing |
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10:00-10:30 |
Gene Annotation II |
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10:30-10:45 |
Break |
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10:45-11:15 |
Gene Annotation II |
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11:15-11:45 |
Mini-Seminar: Transposons |
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11:45-12:30 |
Prospecting Transposons in Genomes - Yellow Line |
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12:30-01:30 |
Lunch |
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01:30-02:30 |
DNA Barcdoing electrophoresis and sequencing prep |
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02:30-03:30 |
DNA Barcoding - Blue Line |
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03:30-03:45 |
Break |
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03:45-04:30 |
Planning and Classroom Implementation |
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04:30-05:00 |
Evaluation and Graduation |
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Other Websites and Links and Resources
- DNA Subway Manual
- Workshop handouts in WORD and PDF
- DNA Barcoding Manual
- Barcoding lab planning and prep
- http://www.learnaboutsma.org/ - SMA website and splicing animation.
- http://www.urbanbarcodeproject.org - UBP website
- http://www.iBoL.org - International Barcode of Life Project
Post Workshop Survey
Please complete this survey at the conclusion of the workshop:
Post Workshop DNA Sequencing Results
Instructions on retrieving your results will be posted here after the workshop.
Important Pre-Workshop Travel Info
Recommended Lodging
There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.
Check website like hotels.com and travelocity.com for additional hotels and rates.
Hotel |
Website |
Notes |
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Homewood Suites Fargo |
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Radisson Hotel Fargo |
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Holiday Inn Fargo |
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Staybridge Suites Fargo |
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Nearest Airports/ Rail
Airport |
Distance |
Notes |
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Fargo Hector International Airport |
~3 Miles |
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Taxi rates and rentals info:
Call ahead of time to reserve/confirm
Service |
Notes |
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From airport website |
Parking Information:
Main campus there is a visitors parking lot right in front of Loftsgard hall. Summer and very flexible, so people can park almost anywhere. It is a weekend and campus police does not ticket people on weekends.