2016 02 01 Genomics in Education Workshop - University of Delaware

2016 02 01 Genomics in Education Workshop - University of Delaware

Organizers:

A. Hlousek-Radojcic, J. Williams

Local Point of Contact:

Alenka Hlousek-Radojcic

Trainers:

Joslynn Lee, Jason Williams

Date:

Feb. 1-2, 2015

Location:

University of Delaware

Newark, DE

Room: Interdisciplinary Science and Engineering Laboratory (ISE) - 410

221 Academy St. University of Delaware

Parking Information/Permits

https://udel.t2hosted.com/cmn/index.aspx.

Pre-Workshop Assignments

Confirm/Establish laptop compatibility:

Preparing your laptop:

  • Most laptops (<5 years old) updated with the latest web browser will have no problem. However, we do strongly recommend using the Firefox Browser (http://www.mozilla.org/en-US/firefox/fx/). Use of Internet Explorer will almost certainly cause problems, while Chrome has caused problems occasionally.Safari (Safari 4 or later) works well.

  •  Try to have administrative privileges on your computer (should it become necessary to install or update software during the workshop).

  •  Several workflows in the current version of DNA Subway require Java. Newer Java versions often limit some functionality in DNA Subway.

  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if a view of the Apollo annotation browser opens up that looks like this screenshot: jpg\/png\/tif\.

  • Should you experience difficulties, follow these steps:

    • Ensure that your computer is running the latest Java by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version.

    • If you are not running the latest Java, install or update it. (If you are a Mac user and experience trouble with this, contact williams@cshl.edu for additional advice).

    • Still having trouble opening Apollo? Open the Java settings (Control Panel or Systems Preferences), click Security tab. Add the following URLs to the Exception Site List:

    • If Java still does not open, enter these URLs into the Exception Site List as well. (One at a time.)

    • If none of the above worked, get Java 6 at this link:* http://java.com/en/download/manual_v6.jsp, and install it on your laptop.

Make sure you have a valid iPlant account:

Go through these readings and other assignments:

  • Review DNA Subway manual (a printed copy will be presented at the workshop)

  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)

  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.

  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 

  • Check out the slides and papers listed/linked in the agenda below.

 

Workshop Agenda and Resources

Thursday

Room

Thursday

Room

Time

Topic

Slides

Notes/Links

08:30-9:00

Arrive / Set up Computers

 

 

09:00-09:15

Welcome/Logistics

 

 

09:15-09:45

Introductions

 

 

09:45-10:00

iPlant Collaborative & workshop objectives

iPlant & Workshop Overview

iPlant Homepage
iPlant Account Portal

10:00-10:15

DNA Subway tour

 

DNA Subway Homepage
DNA Subway Manual

10:15-10:30

Break

 

 

10:30-12:00

Using the Red Line & DNA Annotation to Teach Concepts in Molecular Biology

Red Line Annotation

Concepts and Links
Genomes as Information

Red Line Walk-through
Paper: Gene Annotation

GFX DNALC Server

12:00-01:00

Lunch

 

 

01:00-01:15

Mini Seminar: Evidence Based Gene Modeling

Evidence

 

01:15-02:30

Annotate Genes I: On Your Own

 

ENCODE Project
ENCODE Nature

Ensembl video on genome patches and haplotypes

02:30-02:45

Break

 

 

02:45-03:00

Mini-Seminar: RNA Splicing

Splicing

Alternative Splicing Paper

03:00-04:00

Annotate Genes II: User-BLAST

 

"User Evidence:" EST data
Additional EST data: http://gfx.dnalc.org/files/evidence/Annotation/

04:00-05:00

Demonstration: RNA-Seq and Green Line

RNA-Seq

 

Friday

 

 

 

Time

Topic

Slides

Notes/Links

09:00-09:30

Review, Open Questions From Day 1

 

 

09:30-9:45

Mini Seminar: Transposons

Transposons

 

09:45-10:30

Find Transposons in a Genome (Yellow Line)

 

Yellow Line Walk-through
Paper: TARGeT
Paper: Ping Transposon Burst in Rice Tissue Culture

Paper: The Necessary Junk

Slides: Genome Evolution Propels Evolution of Function

10:30-10:45

Break

 

 

10:45-12:00

DNA Barcoding - Isolate DNA & Prep PCR

 

DNA Barcoding Protocol

12:00-01:00

Lunch

 

 

01:00-01:15

Mini Seminar: DNA Barcoding and Student Projects

DNA Barcoding

Urban Barcode Project

01:15-02:15

DNA Barcoding - Electrophoresis and Prep DNA for Sequencing

 

 

02:15-02:30

Break

 

 

02:30-03:30

Analyze DNA Barcodes (Blue Line)

 

Blue Line Walk-through

03:30-03:45

Evaluation, and Closure

 

Survey Link: https://www.surveymonkey.com/s/GenomicsInEducation_post

Other Workshop Exchanges

Other Websites and Links and Resources

Attendees

First name

Last name

Research Area

Email

First name

Last name

Research Area

Email

Behnam

Abasht

Genetics and Nucleic Acids

abasht@UDel.edu

Dylan

Audette

Developmental Biology

audetted@udel.edu

Amy

Biddle

Other

asbiddle@udel.edu

Jose Daniel

Chazi Capelo

Computer and Computation Research

jdchc@udel.edu

Trevor

Daly

Chemistry

tdaly@udel.edu

Weixuan

Fu

Systematic and Population Biology

wfu@udel.edu

Jacqueline

Hilsenbeck-Fajardo

Chemistry

jfajardo@udel.edu

Seth

Hunt

Ecological Studies

sethhunt@udel.edu

John R

Jungck

Molecular Biosciences

jungck@udel.edu

Terry

Kamps

Genetics and Nucleic Acids

tkamps@njcu.edu

SABARINATH

NEERUKONDA

Cell Biology

nnvsnath@udel.edu

Carl

Schmidt

Biochemistry and Molecular Structure and Function

schmidtc@udel.edu

Marcia

Shofner

Ecological Studies

mshofner@umd.edu

Juan Paolo

Sicat

Training

sicat.paolo20@gmail.com

Armando

Solis

Biochemistry and Molecular Structure and Function

asolis@citytech.cuny.edu

Anne

Terrell

Science and Engineering Education

aterrell@udel.edu

Joseph

Tomlinson

Genetics and Nucleic Acids

mjtiv@udel.edu

Jolie

Wax

Other

jwax@gmail.com

Randy

Wisser

Plant Biology

rjw@udel.edu

Renate

Wuersig

 

rwuersig@udel.edu

Barcoding samples and Gel Images

 

Name

Sample Number (for sequencing)

Bag Number

Species name (if known)

Name

Sample Number (for sequencing)

Bag Number

Species name (if known)

Seth Hunt

1

11

Equisetum

Seth Hunt

2

 

Tradescantia zebrina

Jacqueline Fajardo

3

15

Vinca

Jacqueline Fajardo

4

17

?

Alenka Hlousek-Radojcic

5

2

ISE Lab

Alenka Hlousek-Radojcic

6

8

ISE Lab

Terry Kamps

7

7

?

Terry Kamps