2014 03 26 Mission Critical Bioinformatics

2014 03 26 Mission Critical Bioinformatics

Organizers

Monica Britton, Ian Korf, Jason Williams

iPlant Trainers

Jason Williams, Roger Barthelson

UC Davis Trainers

Mike Covington, Joe Fass, Monica Britton, Sebastian Reyes Chin-Wo, Raj Nandety, Aashish Ranjan, Michelle Stitzer

Date

March 25-27, Webinar Follow up: April 3rd

Location

UC Davis
Sci Lab 2060

Workshop Checklist

1. iPlant Account

2. Atmosphere Access

3. Uploaded Data (Optional)

If you are bringing data to the workshop, please follow the instructions below. It is recommended to upload your data before attending the workshop. You should upload from the best possible internet connection, and if you have any issues email support@iplantcollaborative.

Depending on your level of comfort and the size of the data, you may want to try one of two methods.

1)   iDrop – a desktop drag/drop utility

2)   iCommands – command line interface 

For iDrop, you can download the utility here: bit.ly/1i7wm4s

For iCommands, you can download the scripts here: bit.ly/1kNdDyR

When uploading via iCommands (using iput) it is recommended that you use the T option to renew the connection socket, especially for single large (>30GB) files. The documentation also discusses the options to display progress and to create a restart file. 

Once you have uploaded the data, please fill out this survey (https://docs.google.com/forms/d/1DdQMjk_ri9L4eEma5AOLiRsGMOPRwOB0mVezThF_W2o/viewform ) so that we can have a clearer idea of what data you will be bringing.

4. Laptop (Optional)

You may wish to bring your own WiFi enabled laptop to the workshop. We will be working from the computer lab, but you may choose to use your own laptop either during the course, or for homework. Please be sure your laptop has the following:

Agenda

This agenda is an outline of the activities we will present over the 2 days of the workshop. We will make every effort to make adjustments as needed based upon the needs and interests of the participants.

Downloads:

Handout

March 26th

Time

Activity

Instructor

Slides

Links

09:00

Introduction and iPlant Overview

Jason

Slides

 

09:20

Intro to the Discovery Environment

Aashish

DE Overview March 2014.pptx

 

09:50

Overview of the Data Store

Jason

Slides

 

10:20

Break

 

 

 

10:30

Introduction to Atmosphere (cmd line)

Michael

 

 

11:00

Topic I/II: Introduction to RNA-Seq w/wo reference genomes

Monica and Aashish

MB_RNASeq_March_2014.pdf 
MB_RNASeq_Tuxedo_March_2014.pdf

 

12:00

Lunch

 

 

 

01:00

Group Breakouts - Get jobs started

 

 

 

01:30

Topic III: de novo transcriptome assembly and RNA-Seq

Aashish and Michelle

Transcriptome_assembly_RNAseq.pptx

 

02:20

Group Breakouts - Continue on jobs and/or try transcriptome demos

 

 

 

???

Downtime talk - QC, file formats, and other topics in RNA-Seq

Joe

NGS-talk-JF.pdfQAI-talk-JF.pdfTueAM-alignment-JF.pdf

 

4:30

Wrap-up and homework goals

 

Jason's RNA-Seq Slides

iPlant Learning Center RNA-Seq Tutorial - Tuxedo

March 27th

Time

Activity

Instructor

Slides

Links

09:00

Group homework check

 

 

 

09:30

Topic IV: Variant calling

Michael

 

https://gist.github.com/mfcovington/9811551

10:00

Group Breakouts - continue existing jobs and/or try variant calling demo

 

cummeRbund Slides

RNA-Seq with cummeRbund

10:30

Break

 

 

 

11:00

Topic V: Genome Annotation - MAKER

Sebastian

iPlant_workshop_GenomeAnnotation.pptx

Wiki Maker-P tutorial

11:30

Group Breakouts - continue existing jobs and/or try MAKER

 

 

 

12:00

Lunch

 

 

 

01:00

Topic VI: Genome/ Amplicon Assembly

Joe

assembly-lecture-JF.pdf

 

01:30

Group Breakouts and additional topics as needed

 

 

 

03:30

Topic VII: How to get help - discussion on forums, networking, 
Roadmapping: Work on individual participant goals for virtual follow up

All

 

 

Workshop Evaluation: Please click here!

Command Line Resources

Software Carpentry - Great slides and comprehensive instructions for command line, python, etc. (See: the UNIX shell section)

Westwind - MAC specific, but very well explained and most of the commands work on any Linux system

ss64 - Comprehensive glossary and help