2014 03 26 Mission Critical Bioinformatics
Organizers |
Monica Britton, Ian Korf, Jason Williams |
---|---|
iPlant Trainers |
Jason Williams, Roger Barthelson |
UC Davis Trainers |
Mike Covington, Joe Fass, Monica Britton, Sebastian Reyes Chin-Wo, Raj Nandety, Aashish Ranjan, Michelle Stitzer |
Date |
March 25-27, Webinar Follow up: April 3rd |
Location |
UC Davis |
Workshop Checklist
1. iPlant Account
- Get your free account at http://user.iplantcollaborative.org.
2. Atmosphere Access
- Once you have your account, go to https://user.iplantcollaborative.org/dashboard/ and under Available Services, request access to Atmosphere. Indicate that you are attending a workshop when you are asked for justification.
3. Uploaded Data (Optional)
If you are bringing data to the workshop, please follow the instructions below. It is recommended to upload your data before attending the workshop. You should upload from the best possible internet connection, and if you have any issues email support@iplantcollaborative.
Depending on your level of comfort and the size of the data, you may want to try one of two methods.
1) iDrop – a desktop drag/drop utility
2) iCommands – command line interface
For iDrop, you can download the utility here: bit.ly/1i7wm4s
- You can read documentation here: bit.ly/PuWXPP
- You can see a video explanation here: http://youtu.be/0YiMG3OMbhM
For iCommands, you can download the scripts here: bit.ly/1kNdDyR
- You can read documentation here: bit.ly/1frB8W1
- You can see a video explanation here: http://youtu.be/C8tLzBdNG8Y
When uploading via iCommands (using iput) it is recommended that you use the T option to renew the connection socket, especially for single large (>30GB) files. The documentation also discusses the options to display progress and to create a restart file.
Once you have uploaded the data, please fill out this survey (https://docs.google.com/forms/d/1DdQMjk_ri9L4eEma5AOLiRsGMOPRwOB0mVezThF_W2o/viewform ) so that we can have a clearer idea of what data you will be bringing.
4. Laptop (Optional)
You may wish to bring your own WiFi enabled laptop to the workshop. We will be working from the computer lab, but you may choose to use your own laptop either during the course, or for homework. Please be sure your laptop has the following:
- VNC Viewer: Download the DMG (for MAC) or exe (for PC): http://www.realvnc.com/download/viewer/
- Java: Please have JAVA installed and enabled (help)
- Browser: Please have an up-to-date web browser (Recommended Firefox or Safari )
Agenda
This agenda is an outline of the activities we will present over the 2 days of the workshop. We will make every effort to make adjustments as needed based upon the needs and interests of the participants.
Downloads:
March 26th
Time |
Activity |
Instructor |
Slides |
Links |
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09:00 |
Introduction and iPlant Overview |
Jason |
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09:20 |
Intro to the Discovery Environment |
Aashish |
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09:50 |
Overview of the Data Store |
Jason |
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10:20 |
Break |
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10:30 |
Introduction to Atmosphere (cmd line) |
Michael |
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11:00 |
Topic I/II: Introduction to RNA-Seq w/wo reference genomes |
Monica and Aashish |
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12:00 |
Lunch |
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01:00 |
Group Breakouts - Get jobs started |
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01:30 |
Topic III: de novo transcriptome assembly and RNA-Seq |
Aashish and Michelle |
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02:20 |
Group Breakouts - Continue on jobs and/or try transcriptome demos |
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|
??? |
Downtime talk - QC, file formats, and other topics in RNA-Seq |
Joe |
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4:30 |
Wrap-up and homework goals |
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March 27th
Time |
Activity |
Instructor |
Slides |
Links |
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09:00 |
Group homework check |
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09:30 |
Topic IV: Variant calling |
Michael |
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10:00 |
Group Breakouts - continue existing jobs and/or try variant calling demo |
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10:30 |
Break |
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11:00 |
Topic V: Genome Annotation - MAKER |
Sebastian |
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11:30 |
Group Breakouts - continue existing jobs and/or try MAKER |
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12:00 |
Lunch |
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01:00 |
Topic VI: Genome/ Amplicon Assembly |
Joe |
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01:30 |
Group Breakouts and additional topics as needed |
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03:30 |
Topic VII: How to get help - discussion on forums, networking, |
All |
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Workshop Evaluation: Please click here!
Command Line Resources
Software Carpentry - Great slides and comprehensive instructions for command line, python, etc. (See: the UNIX shell section)
Westwind - MAC specific, but very well explained and most of the commands work on any Linux system
ss64 - Comprehensive glossary and help