2012 08 13 - Genomics in Education Workshop, Bozeman MT

2012 08 13 - Genomics in Education Workshop, Bozeman MT

Organizers:

Jason Williams and Mari Eggers

Local Point of Contact:

Mari Eggers

Trainers:

David Micklos, Mohammed Khalfan

Date:

August 13-14th, 2012

Location:

Montana State University, Bozeman, MT
Room 233 of the Strand Union Building 

Section Navigation

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 13/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get Java 6 @ this link: http://java.com/en/download/manual_v6.jsp
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • ReviewDNA Subway manual (a printed copy will be presented at the workshop)
  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Wiki Short URL: http://www.iplantcollaborative.org/bozwiki

Monday, August 13th

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

Overview, Workshop Objectives, DNA Subway Tour


 

10:15-10:30

iPlant Collaborative Overview

iPlant Intro.ppt

 

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to Teach Concepts in Molecular Biology

1 Intro and Red Line.pptx

 

11:15-12:00

Setting up projects for Annotation - Red Line

 

 

12:00-01:00

Lunch

 

 

01:00-01:15

DNA Barcoding setup

 

 

01:15-01:30

Mini-Seminar: DNA Barcoding

2 Barcoding and Blue Line.pptx

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:45

Gene Annotation I

 

 

04:45-05:00

Mini-Seminar: Alternative Splicing                                                      

 

 

Tuesday, August 14th

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              

Plant transposons.ppt

 

09:30-10:15

Transposon Sleuthing: Yellow Line

 

 

10:15-10:30

Break

 

 

10:30-11:30

Gene Annotation II

 

 

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch

 

 

01:30-02:30

DNA Barcoding - Blue Line

 

 

02:30-02:45

Break

 

 

02:45-03:45

Barcdoing electrophoresis and sequencing prep

 

 

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

Post Workshop DNA Sequencing Results

Instructions on retrieving your results will be posted here after the workshop.

Important Pre-Workshop Travel Info

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Check website like hotels.com and travelocity.com for additional hotels and rates. 

Here is a link from the MSU website for accommodations: http://www.montana.edu/accommodations/

Nearest Airports/ Rail

Airport

Notes

Bozeman Yellowstone International Airport (BZN)


Ground Transportation info:

Call ahead of time to reserve/confirm

Parking Information:

You can park in campus parking on 7th Ave. (See “P” for parking on campus map.) The fee is $2.50/day to park in this lot.

On-Campus Directions:

A campus map of MSU can be found at this link: http://www.montana.edu/campusmap