2012 10 30 - Genomics in Education Workshop, University of Alaska-Anchorage, AK

2012 10 30 - Genomics in Education Workshop, University of Alaska-Anchorage, AK

Organizers:

Jason Williams

Local Point of Contact:


Trainers:


Date:

October 30th - 31st, 2012

Location:

University of Alaska, Anchorage, AK

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 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 13/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get Java 6 @ this link: http://java.com/en/download/manual_v6.jsp
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Screenshot

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • ReviewDNA Subway manual (a printed copy will be presented at the workshop)
  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Wiki Short URL: http://www.iplantcollaborative.org/akwiki

Tuesday, October 30th

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

Overview, Workshop Objectives, DNA Subway Tour

New DNA Subway workshop introduction1.ppt

 

10:15-10:30

iPlant Collaborative Overview

iPlant_intro.ppt

 

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to Teach Concepts in Molecular Biology

Red line lesson sketch.ppt

 

11:15-12:00

Setting up projects for Annotation - Red Line

 

 

12:00-01:00

Lunch

 

 

01:00-01:15

DNA Barcoding setup

 

 

01:15-01:30

Mini-Seminar: DNA Barcoding

plantdnabarcoding.ppt

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:45

Gene Annotation I

 

 

04:45-05:00

Mini-Seminar: Alternative Splicing                                                      

alternative splicing.ppt

 

Wednesday, October 31st

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              

Plant transposons.ppt

 

09:30-10:15

Transposon Sleuthing: Yellow Line

Green Revolution.ppt

 

10:15-10:30

Break

 

 

10:30-11:30

Gene Annotation II

 

 

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch

 

 

01:30-02:30

DNA Barcoding - Blue Line

Blue_line_phylo_intro.ppt

 

02:30-02:45

Break

 

 

02:45-03:45

Barcdoing electrophoresis and sequencing prep

 

 

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

Post Workshop DNA Sequencing Results

Instructions on retrieving your results will be posted here after the workshop.

Instructions for accessing your DNA Sequence from DNA Subway

 

1.       Log into DNA Subway (dnasubway.iPlantcollaborative.org)

2.       Start a blue line project

3.       Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding

4.       Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number:

10-209384819

and are posted as the entry under Jermel Watkins on 11/7/12

5.       Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.

           For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....

Gel Photo

Important Pre-Workshop Travel Info

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Check website like hotels.com and travelocity.com for additional hotels and rates. 

Hotel

Website

Notes




Nearest Airports/ Rail

Airport

Distance

Notes




Taxi rates and rentals info:

Call ahead of time to reserve/confirm

Service

Notes



Parking Information:

On-Campus Directions: