2012 06 29 TSW NDSU

2012 06 29 TSW NDSU

Organizers:

411, Sheldon McKay, Jason Williams

Local Point of Contact:

Shahryar F. Kianian

Trainers:

Sheldon McKay, Christos Noutsos, Shannon OliverJason Williams

Date:

12:00PM - 6:00PM on June 29, 9:30AM - 4:00PM on June 30, 2012

Location:

Barry Hall, Rm 260

Goal

To provide an interactive, hands-on training of iPlant Tools and Services so that participants can begin to use iPlant cyberinfrastructure in their research.

Pre-Workshop Homework

1. Please test your iPlant credentials to ensure you have access. You can log-in from user.iplantcollaborative.org, click the button "iPlant Login"

  • Once authenticated, click the "My Services" link
  • Under "My Services" please ensure you have access to: Atmosphere, Discovery Environment, iPlant Data Store. You can also request access to other services here
  • If you have account problems, please email support@iplantcollaborative.org

2. Please check your email and this wiki prior to the workshop, as this is the way we will communicate any important changes.

Workshop Notes

Here are a few important links that you may wish to refer to during and after the workshop:

Account/Password Problems

iPlant Help and Discussion Forums

General iPlant Support Issues

iPlant Privacy (And other) policies - "Your Data are Your Data"

Agenda

Friday June 29th 

Time

Presentation

Audience/Prerequisites*

Presenter

Slides, Links

12:00 PM - 12:30 PM 

Arrive / Sign-in / Verify iPlant Accounts

N/A

 

 

12:30 PM - 01:00 PM

Welcome - Presenter/Participant Introductions

N/A

Jason Williams

 

01:00 PM - 01:20 PM

An Overview of the iPlant Collaborative

All researchers

Jason Williams

iPlant intro_fargo.pptx

01:20 PM - 01:45 PM

Overview of the iPlant Discovery Environment (DE)

All researchers

Jason Williams

Overview of DE

01:45 PM - 02:15 PM

iPlant Data Store - Managing "Big Data" 

All researchers

Jason Williams

Data Store Overview
/wiki/spaces/eot/pages/241585216

02:15 PM - 02:30 PM

Break

 


 

02:30 PM - 03:00 PM

Building and Using Workflows Within the DE; Phylogenetics 

All researchers

Sheldon McKay

intro_phylo_workflow.pptx

03:00 PM - 04:15 PM

Using the DE to Examine Differential Expression Within an RNA-seq Dataset

All researchers - Background in
RNA-Seq methods is helpful

Sheldon McKay

Indroductory_RNA-seq.ppt.
/wiki/spaces/eot/pages/241585220
Useful Article

04:15 PM - 4:30 PM

Break

 

 

 

04:30 PM - 05:00 PM

Atmosphere - Custom Cloud Computing

All researchers - Background in
Linux computing helpful

Christos Noutsos

 Atmosphere Overview

05:00 PM - 06:00 PM

Extending the DE for Your Research: Tool Integration and Customization

Intermediate - Background in
bioinformatics/ programming

Sheldon McKay

 

06:00 PM - 06:15 PM

Collaborating with iPlant: Future Projects and Workshop Summary 

All researchers

Sheldon McKay

Workshop Conclusion.pptx

Saturday June 30th 

Time

Presentation

Audience/Prerequisites*

Presenter

Slides/Links

09:30 AM - 09:40 AM

Installing Java

All of you

 

 

09:40 AM - 11:00 AM

Advanced CHiP-seq in the iPlant Cyberinfrastructure

Intermediate - knowledge of
iPlant Discovery Environment,
CHiP-Seq methods helpful.

Sheldon McKay

AdvancedChIPseq.ppt
/wiki/spaces/eot/pages/241585258

11:00 AM - 11:15 AM

Break

 

 

 

11:15 AM - 12:00 PM

Topics and Questions in RNA-Seq

All researchers

Sheldon McKay

/wiki/spaces/eot/pages/241585220

12:00 PM - 01:00 PM

Lunch

 

 

 

01:00 PM - 01:15

Upcoming Features in the DE

All

Shannon Oliver

 

01:15 PM - 01:30 PM

Using XSEDE for Bioinformatics 

Advanced- Background
in Bioinformatics and HPC

Jason Williams

XSEDE Slides

01:30 PM - 02:00 PM

Powered by iPlant - Using the iPlant API 

Intermediate/Advanced - Background
in bioinformatics/ programming

Jason Williams

API Tutorial
API Slides
Jason's Cheat Sheet

02:00 PM - 02:45 PM

iCommands for data transfer

Intermediate/Advanced Background in command line
Linux programming helpful

Christos Noutsos

 

02:45 PM - 03:45 PM

Using Atmosphere to visualize RNA-Seq data

Intermediate/Advanced- Background in RNA-Seq
analysis helpful

Sheldon McKay

/wiki/spaces/eot/pages/241585166
cummeRbund Manual

03:45 PM

Conclusion and Free Form Work

All audiences as needed.

 

 

* Audience/Prerequisites - These prerequisites are meant as helpful suggestions to get the most out of each module. Everyone can attend

any/all modules and are free to follow along (or not) as their own interests and abilities dictate.

Attendee List

First

Last

Email

User Acct

Omar

Al-Azzam

omar.al-azzam@ndsu.edu

X

Aman

Anand

aman.anand@my.ndsu.edu

no acct

Deepika

Arora

deepika.arora@my.ndsu.edu

X

Filippo

Bassi

filippo.bassi@ndsu.edu

X

Ryan

Burciaga

ryan.burciaga@my.ndsu.edu

X

Anne

Denton

anne.denton@ndsu.edu

X

Sandra

Dunckel

sdunckel@ksu.edu

needs Atmo

Morgan

Echeverry-Solarte

morgan.echeverry-solarte@my.ndsu.edu

X

Ahmed

Eldoliefy

ahmed.el-doleify@my.ndsu.edu

needs Atmo

Farhad

Ghavami

farhad.ghavami@ndsu.edu

needs Atmo

David

Horvath

david.horvath@ars.usda.gov

X

Shalu

Jain

shalu.jain@ndsu.edu

needs Atmo

Renata

Jung

renata.jung@ndsu.edu

needs Atmo

Vedbar

Khadka

vskhadka@jacks.sdstate.edu

needs Atmo

Shahryar

Kianian

s.kianian@ndsu.edu

needs Atmo

Ajay

Kumar

ajayguptajammu@gmail.com

needs Atmo

Cindy

Lawley

clawley@Illumina.com

X

Yueqiang

Leng

yueqiang.leng@gmail.com

X

Guojia

Ma

guojia.ma@ndsu.edu

needs Atmo

Samira

Mafi Moghaddam

samira.mafi@ndsu.edu

X

Sujan

Mamidi

sujan.mamidi@gmail.com

X

Mona

Mazaheri

mona.mazaheri@ndsu.edu

needs Atmo

Steven

Meinhardt

steven.meinhardt@ndsu.edu

X

Monika

Michalak de Jimenez

monika.michalak@ndsu.edu

no acct

Sepehr

Mohajeri Naraghi

sepehr.mohajerinarag@my.ndsu.edu

no acct

Andres

Noyszewsici

anoyszew@umn.edu

X

Krishna

Puri

krishna.puri@ndsu.edu

X

Andres

Salcedo

asalcedo@ksu.edu

X

Raed

Seetan

raed.seetan@ndsu.edu

X

Santosh

Sharma

santosh.sharma@my.ndsu.edu

X

Gongjun

Shi CANCELLED

gongjun.shi@ndsu.edu

needs Atmo

Ricardo

Silveira

ricardo_biologia@hotmail.com

needs Atmo

Marek

Sliwinski

marek.k.sliwinski@uni.edu

X

Kristin

Simons

kristinsimons77@hotmail.com

X

Ali

Soltani

Ali.Soltani@my.ndsu.edu

needs Atmo

Qun

Sun

qunsun0710@gmail.com

needs Atmo

Prabin

Tamang

probin.tamang@ndsu.edu

needs Atmo

Vijay K

Tiwari

VIJTIWARI@GMAIL.COM

needs Atmo

Rui

Wang

rui.wang@ndsu.edu

X

Zengcui

Zhang

zengcui.zhang@ndsu.edu

X

Post-Workshop Survey

iPlant is by, for and of the community. It is extremely important for us to hear from you about what works and what doesn't in our training workshops. We'd also like to know about computational bottlenecks you've encountered in your research for which iPlant could develop community-scale solutions. We want to know how you found out about the workshop, and what you would like to see iPlant do, so please respond to the survey you will receive by email after the workshop has been completed.

Parking Information:

On-Campus Directions:

http://www.ndsu.edu/ndsu/maps/CampusMap_2pg.pdf

URLS

 

FTP Link for Demo

1

ftp://ftp.ensembl.org/pub/release-67/fasta/canis_familiaris/dna/Canis_familiaris.BROADD2.67.dna_rm.chromosome.MT.fa.gz

2

ftp://ftp.ensembl.org/pub/release-67/fasta/canis_familiaris/dna/Canis_familiaris.BROADD2.67.dna_rm.chromosome.MT.fa.gz

3

ftp://ftp.ensembl.org/pub/release-67/fasta/danio_rerio/dna/Danio_rerio.Zv9.67.dna_rm.chromosome.MT.fa.gz

4

ftp://ftp.ensembl.org/pub/release-67/fasta/danio_rerio/dna/Danio_rerio.Zv9.67.dna_rm.chromosome.MT.fa.gz

5

ftp://ftp.ensembl.org/pub/release-67/fasta/homo_sapiens/dna/Homo_sapiens.GRCh37.67.dna_rm.chromosome.MT.fa.gz

6

ftp://ftp.ensembl.org/pub/release-67/fasta/homo_sapiens/dna/Homo_sapiens.GRCh37.67.dna_rm.chromosome.MT.fa.gz

7

ftp://ftp.ensembl.org/pub/release-67/fasta/mus_musculus/dna/Mus_musculus.NCBIM37.67.dna_rm.chromosome.MT.fa.gz

8

ftp://ftp.ensembl.org/pub/release-67/fasta/mus_musculus/dna/Mus_musculus.NCBIM37.67.dna_rm.chromosome.MT.fa.gz

9

ftp://ftp.ensembl.org/pub/release-67/fasta/gallus_gallus/dna/Gallus_gallus.WASHUC2.67.dna_rm.chromosome.MT.fa.gz

10

ftp://ftp.ensembl.org/pub/release-67/fasta/gallus_gallus/dna/Gallus_gallus.WASHUC2.67.dna_rm.chromosome.MT.fa.gz

11

ftp://ftp.ensembl.org/pub/release-67/fasta/gorilla_gorilla/dna/Gorilla_gorilla.gorGor3.1.67.dna_rm.chromosome.MT.fa.gz

12

ftp://ftp.ensembl.org/pub/release-67/fasta/gorilla_gorilla/dna/Gorilla_gorilla.gorGor3.1.67.dna_rm.chromosome.MT.fa.gz

13

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/arabidopsis_lyrata/dna/Arabidopsis_lyrata.Araly1.12.dna_rm.nonchromosomal.fa.gz

14

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/arabidopsis_lyrata/dna/Arabidopsis_lyrata.Araly1.12.dna_rm.nonchromosomal.fa.gz

15

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/arabidopsis_thaliana/dna/Arabidopsis_thaliana.TAIR10.12.dna_rm.chromosome.Pt.fa.gz

16

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/arabidopsis_thaliana/dna/Arabidopsis_thaliana.TAIR10.12.dna_rm.chromosome.Pt.fa.gz

17

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/brachypodium_distachyon/dna/Brachypodium_distachyon.Brachy1.0.12.dna_rm.nonchromosomal.fa.gz

18

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/brachypodium_distachyon/dna/Brachypodium_distachyon.Brachy1.0.12.dna_rm.nonchromosomal.fa.gz

19

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/glycine_max/dna/Glycine_max.1.0.12.dna_rm.nonchromosomal.fa.gz

20

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/glycine_max/dna/Glycine_max.1.0.12.dna_rm.nonchromosomal.fa.gz

21

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/oryza_sativa/dna/Oryza_sativa.MSU6.12.dna_rm.chromosome.Pt.fa.gz

22

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/oryza_sativa/dna/Oryza_sativa.MSU6.12.dna_rm.chromosome.Pt.fa.gz

23

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/zea_mays/dna/Zea_mays.AGPv2.12.dna_rm.chromosome.chloroplast.fa.gz

24

ftp://ftp.gramene.org/pub/gramene/CURRENT_RELEASE/data/fasta/zea_mays/dna/Zea_mays.AGPv2.12.dna_rm.chromosome.chloroplast.fa.gz

25

ftp://ftp.ncbi.nlm.nih.gov/genomes/Apis_mellifera/CHR_MT/ame_ref_Amel_4.5_chrMT.fa.gz

26

ftp://ftp.ncbi.nlm.nih.gov/genomes/Apis_mellifera/CHR_MT/ame_ref_Amel_4.5_chrMT.fa.gz

27

ftp://ftp.ncbi.nlm.nih.gov/genomes/Glycine_max/CHR_Pltd/gma_ref_V1.0_chrPltd.fa.gz

28

ftp://ftp.ncbi.nlm.nih.gov/genomes/Glycine_max/CHR_Pltd/gma_ref_V1.0_chrPltd.fa.gz

29

ftp://ftp.ncbi.nlm.nih.gov/genomes/Drosophila_melanogaster/RELEASE_5_41/CHR_Un/NS_000188.frn

30

ftp://ftp.ncbi.nlm.nih.gov/genomes/Drosophila_melanogaster/RELEASE_5_41/CHR_Un/NS_000188.frn

31

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_001319.faa

32

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_001319.faa

33

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_000932.fna

34

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_000932.fna

35

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_001603.faa

36

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_001603.faa

37

ftp://ftp.ncbi.nlm.nih.gov/genomes/Chloroplasts/plastids/NC_001603.faa

38

ftp://hgdownload.cse.ucsc.edu/goldenPath/apiMel1/database/all_est.txt.gz

39

ftp://hgdownload.cse.ucsc.edu/goldenPath/apiMel1/database/all_mrna.sql

40

ftp://hgdownload.cse.ucsc.edu/goldenPath/apiMel1/database/all_mrna.sql

41

ftp://hgdownload.cse.ucsc.edu/goldenPath/bosTau1/database/all_mrna.sql

42

ftp://hgdownload.cse.ucsc.edu/goldenPath/bosTau1/database/all_mrna.sql