2014 07 27 Botany 2014 DNA Subway Workshop

2014 07 27 Botany 2014 DNA Subway Workshop

Organizers:

Jason Williams

Local Point of Contact:

Johanne Stogran

Trainers:

Jason Williams, Uwe Hilgert

Date:

July 27th

Location:

Salmon Room,
The Boise Centre - Boise, Idaho

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser will have no problem. However, we do strongly recommend using the Firefox Browser (http://www.mozilla.org/en-US/firefox/fx/). Use of Internet Explorer will almost certainly cause problems, while Chrome has caused problems occasionally.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).

  • Ensure that your computer is running a current browser (Mozilla Firefox 13/ Safari 4 or later).

  • Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get either Java 6 from this link: http://java.com/en/download/manual_v6.jsp, or prime your Java installation as described on Page 5 in the Red Line Walkthrough.

  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.

  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).

  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Again, you may need to prime your Java installation as described above.

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • Review DNA Subway manual (a printed copy will be presented at the workshop)

  • Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)

  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.

  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 

  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Time

Topic

Slides

Notes/Links

Time

Topic

Slides

Notes/Links

08:00-08:15

Welcome/Logistics

 

 

08:15-08:30

Introductions

 

 

08:30-08:45

iPlant Collaborative & workshop objectives

iPlant Intro
Workshop Overview

iPlant Homepage
iPlant account portal

08:45-09:00

DNA Subway tour

 

DNA Subway Homepage
DNA Subway Manual

09:00-10:30

DNA Barcoding intro and Lab

Plant DNA Barcoding

DNA Barcoding 101 site

10:30-10:45

Break

 


10:45-11:30

Analyze a DNA contig (Red Line)

Annotating DNA

Red Line Walkthough Manual
EST data for Synthetic Contig
GFX DNA Subway Repository
Gene Annotation Paper

11:30-12:00

RNA-Seq with Green Line

 

 

Attendees & DNA Barcoding