2014 07 27 Botany 2014 DNA Subway Workshop
Organizers: | Jason Williams |
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Local Point of Contact: | Johanne Stogran |
Trainers: | Jason Williams, Uwe Hilgert |
Date: | July 27th |
Location: | Salmon Room, |
Pre-Workshop Homework
Laptop Compatibility
If you are bringing your own laptop:
Most laptops (<5 years old) updated with the latest web browser will have no problem. However, we do strongly recommend using the Firefox Browser (http://www.mozilla.org/en-US/firefox/fx/). Use of Internet Explorer will almost certainly cause problems, while Chrome has caused problems occasionally.
Here are some things to check:
If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
Ensure that your computer is running a current browser (Mozilla Firefox 13/ Safari 4 or later).
Be aware, Java 7 has known issues that limit some functionality on DNA Subway. Get either Java 6 from this link: http://java.com/en/download/manual_v6.jsp, or prime your Java installation as described on Page 5 in the Red Line Walkthrough.
If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install.
If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Again, you may need to prime your Java installation as described above.
Make sure you have a valid iPlant account
You should be able to log into the DNA subway at: http://dnasubway.iplantcollaborative.org/.
If you do not have an iPlant account, please get one at: http://user.iplantcollaborative.org/register.
Additional Readings and Assignments
Review DNA Subway manual (a printed copy will be presented at the workshop)
Review DNA Barcoding laboratory (a printed copy will be presented at the workshop)
Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so!
Check out the slides and papers mentioned in the agenda.
Workshop Agenda and Resources
Time | Topic | Slides | Notes/Links |
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08:00-08:15 | Welcome/Logistics |
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08:15-08:30 | Introductions |
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08:30-08:45 | iPlant Collaborative & workshop objectives | ||
08:45-09:00 | DNA Subway tour |
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09:00-10:30 | DNA Barcoding intro and Lab | ||
10:30-10:45 | Break |
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10:45-11:30 | Analyze a DNA contig (Red Line) | Red Line Walkthough Manual | |
11:30-12:00 | RNA-Seq with Green Line |
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