20150719 Bioinformatics Workshop at AZCTE

20150719 Bioinformatics Workshop at AZCTE

Event Organizer

Debra James (AZED)

iPlant Trainers

Uwe Hilgert (BIO5)

TAs

Cheryl Blake (PCC); James De La Rosa (PCC)

Date

July 19, 2015

Time

13:00-17:00 (mas o menos)

Location

Loews Ventana Resort

7000 N Resort Dr, Tucson, AZ 85750

Ventana Canyon-Lab 2 Room

Required Workshop Prep

1. iPlant Account

  • Get your free account at http://user.iplantcollaborative.org. (Make sure to REMEMBER your username and password.) Please register with an institutional email account (e.g. '.edu' or '.org') only these types of email addresses will be granted access to the iPlant cloud service Atmosphere (see next bullet).

2. Laptop

Please bring your own WiFi enabled laptop to the workshop. Make sure your laptop has the following:

  • Internet Browser: Please have an up-to-date web browser (We strongly recommend Firefox or Safari - Internet Explorer and Chrome have Java and other compatibility issues.)

  • Java: Have JAVA installed, enabled and working with Firefox or Safari. (Help)

4. Pre-Workshop Guide and Homework

  • Download and print this manual to get the most out of the workshop.

  • Complete this Pre-Workshop Survey

Etherpad/Chatroom

We will use the following Etherpad for instant & shared communications during the workshop: https://etherpad.mozilla.org/eYOfsFktUW

Instructional Materials

Materials are downloadable/accessible from the Resources column in the Workshop Agenda below.

Workshop Agenda

Time

Description

Objectives

Tools

Sources

Resources

Links

12:30

Doors Open / Sign-in / Set up

 

 

 

This workshop in 20 min. or less

Pre-Survey

01:00

Welcome - Self-Introductions

 

 

 

1:15

Overview

Relatedness By Descent

Mobile Genetic Elements (Transposons)

Polymorphism

Allele

 

 

01:30

Wet lab

  • isolate DNA

  • start PCR

Identify personal genotype at PV92 locus

  • Isolate and amplify DNA

MolBio lab equipment

  • Pipets

  • Centrifuge

  • Thermocycler

MolBio lab supplies

  • Chelex

  • Primers & nucleotides

  • Polymerase

  • N. Anderson @ UA

  • Carolina Biologicals

 

02:30

eLab

  • Part A

  • Part B

Identify PV92 locus in data bases

  • Identify and isolate PCR primer sequences

  • Conduct PCR electronically

  • Predict size of PCR amplicon

 

WWW

  • DNALC site

    • Recipes

  • NCBI site

    • BLAST

    • GenBank

 

03:00

Break

 

 

 

 

 

03:15

eLab

  • Part C

Examine PV92 locus in human genome

  • What chromosome is it on?

  • Is it in a gene?

WWW

  • NCBI site

    • Human Genome Viewer (Mapview)

    • BLAST

 

 

04:00

Wet lab

  • Load gel

Identify personal genotype at PV92 locus

  • Analyze amplified DNA

MolBio lab equipment

  • Gel electrophoresis equipment

  • N. Anderson @ UA

  • Carolina Biologicals

  • Invitrogen E-Gels

 

04:15

eLab

  • Part D

Identify and analyze all states of PV92 known to man

WWW

  • NCBI

    • BLAST

  • BioServers

    • SequenceServer

  • DNA Subway

    • Blue Line

    • MUSCLE

  • Mega

 

Human PV92 locus

 

Bioinformatics

04:45

Wet lab

  • Analyze gels

Identify personal genotype at PV92 locus

  • Analyze agarose gel

Understand population genetics

  • Hardy-Weinberg equilibrium

  • Genetic Drift versus Selective Pressure

  • Human Migration

MolBio lab equipment

  • Lightbox

WWW

  • Allele Server

  • Simulation Server

  • N. Anderson @ UA

  • Carolina Biologicals

  • IO Rodeo

05:00 m.o.m.

Dismissal

 

 

 

 

 

Consultations

Still questions?

 

 

 

 

Participants

Alonso, Martha Celia

Douglas High School; Douglas, AZ

 

Chamberlain, Karen A

Central Arizona Valley Institute of Technology (CAVIT); Coolidge, AZ

 

Cherry, Amanda

Mesa High School; Mesa, AZ

 

DeJesus, Jo

Tuba City High School; Tuba City, AZ

 

Dunn, Erynn

Casa Verde High School; Casa Grande, AZ

 

Ford, Matt

Chino Valley High School; Chino Valley, AZ

 

Griego, Ken

Miami High School; Miami, AZ

 

Hoover, Kathy

KOFA High School; Yuma, AZ

 

Landry, Marni

Paradise Valley High School; Phoenix, AZ

 

Lettes, Andrew

Pueblo Magnet High School; Tucson, AZ

 

Livingston, Michele

Marana High School; Tucson, AZ

 

Lord, Peggy

Stapley Junior High School; Mesa, AZ

 

Scaife, Rick

Valley Vista High School; Surprise, AZ

 

Vreyens, John

Chino Valley High School; Chino Valley, AZ

 

Zeiher, Carolyn

Canyon Del Oro High School; Oro Valley, AZ

 

 

Logistics, Accommodations, and other details

Arrival Time

Please arrive at 12:30 PM for registration to avoid delays to the workshop.