2016 03 31 Tools and Services Workshop - Southern Illinois University Carbondale
Organizers: | C.J. Geisler-Lee |
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Local Point of Contact: | Cheng-Li Jane Heisler-Lee |
Trainers: | Joslynn Lee, Kapeel Chougule, Pan Teng |
Date: | March 31 - April 1, 2016 |
Location: | Life Sciences Building II Room 404 Southern Illinois University Carbondale Carbondale, IL 62901 |
Parking Information/Permits |
Required Workshop Prep
1. CyVerse Account
- Get your free account at http://user.iplantcollaborative.org/ . (Make sure to REMEMBER your username and password.) Please register with an institutional email account as only this will give you access to the CyVerse cloud service Atmosphere (see next bullet). Please avoid gmail, yahoo, hotmail and the like for your CyVerse registration as you would not be able to register for Atmosphere with those.
2. Atmosphere Access
- Once you have your account, go to http://user.iplantcollaborative.org/ and log in. Under "Apps and Services," scroll down to "Available Services" and request access to Atmosphere and DNA Subway. Indicate that you are attending a workshop when you are asked for justification. (This will only be granted if you registered for CyVerse using an institutional account; see above.)
3. Laptop
Please bring your own WiFi enabled laptop to the workshop. Make sure your laptop has the following:
- Internet Browser: Please have an up-to-date web browser (We strongly recommend Firefox or Safari; Internet Explorer, Chrome and others have significant issues.)
- Java: Have JAVA installed, enabled and working with Firefox or Safari. (Help)
- Data Store: Download and install iCommands (iCommands for your OS)
- VNC Viewer: Download the .dmg (for MAC) or exe (for PC) (http://www.realvnc.com/download/viewer/)
- Windows Users only: Download and install PuTTY (http://www.chiark.greenend.org.uk/~sgtatham/putty/download.html)
- Download Cyberduck: Using Cyberduck for Uploading and Downloading to the Data Store - Installing and Configuring Cyberduck
4. Pre-Workshop Guide and Homework
- Download and complete the exercises in the PRE-WORKSHOP PACKET to get the most out of the workshop.
- Complete this Pre-Workshop Survey
Instructional Materials
You can download a copy of the workshop packet (CyVerse Toolkit) to follow along:
Draft Workshop Program (DAY 1)
Time | Description | Toolkit | Slides | Links | Presenter |
08:30 AM | Arrive / Sign-in / Setup Troubleshooting |
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09:00 AM | Welcome - Presenter/Participant Self-Introductions | slides | Joslynn | ||
09:30 AM | Overview of CyVerse | Joslynn | |||
09:45 AM | Managing "Big Data" using the CyVerse Data Store |
| slides | Data Store Overview | Joslynn |
10:30 AM | Coffee Break: Room LSII 460 |
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10:45 AM | Navigate the CyVerse Discovery Environment (DE)
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| slides | Kapeel | |
11:15 AM | Analyses in the DE; Phylogenetics Example |
| Kapeel | ||
12:00 PM | Lunch: LSII 453 |
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01:00 PM | DNA Subway (Workflows for Bioinformatics Education) | slides | DNA Subway Website | Joslynn | |
01:15 PM | Using the DE for RNA-Seq |
| slides | Kapeel | |
02:15 PM | Atmosphere Cloud Computing |
| slides |
| Joslynn |
03:00 PM | Coffee Break: Room LSII 460 |
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03:15 PM | Tool Integration in the DE |
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| Kapeel | |
03:45 PM | Sequence Read Archive Demo |
| slides | Kapeel | |
04:15 PM | Workshop summary and conclusion |
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Draft Workshop Program (DAY 2)
slides
slidesTime | Description | Slides | Links | Presenter |
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09:30 AM | Transcriptome assembly (TA) with Trinity and quantification with Kallisto tools | slides (TA) slides (kallisto) | Kapeel | |
10:30 AM | Coffee Break: Room LSII 460 |
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10:45 AM | Getting started with Annotation: MAKER (MP) |
| Kapeel | |
11:15 AM | Visualization of RNA-Seq results in Atmosphere | slides | ||
12:00 PM | Lunch (on your own) |
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01:00 PM | iCommands and other Data Store applications: | Joslynn | ||
01:30 PM | “What Graduate Students should Know about Cyberinfrastructure | Pan | ||
02:30 PM | Individual consultations |
| Please posts your requests for 10-15 min |
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Links to paper/tools/resources
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Cole Trapnell, Adam Roberts, Loyal Goff, Geo Pertea, Daehwan Kim et al.
Nature Protocols Mar 1, 2012
Sleuth http://pachterlab.github.io/sleuth/about.html
Important Pre-Workshop Travel Info
Neither CyVerse/iPlant Collaborative nor the local host can reimburse lodging or other expenses associated with attending the workshop.
Maps and parking information can be found here: http://parking.siu.edu/on-campus/index.html
Visitor Information: http://admissions.siu.edu/visit/visitor-information.php
Travel/Lodging
See the city of Carbondale, IL website: http://www.carbondaletourism.org/categories/stay