2012 04 26 Genomics in Education Workshop, Southern University at New Orleans

2012 04 26 Genomics in Education Workshop, Southern University at New Orleans

Organizers:

Jason Williams and Sheldon McKay

Local Point of Contact:

Solomon Adekunle

Trainers:

Jason Williams and Sheldon McKay

Date:

April 26-27

Location:

Southern University at New Orleans, Park Campus, 6400 Press Drive, New Orleans, LA 70126
New Science Building (NSB), 302

Section Navigation

 Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 3.6/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install the newest version.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot: Java Test.pdf

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • Review the DNA Subway Manual (a printed copy and some other handouts will be presented to you at the workshop).
  • Review the DNA Barcoding wet-lab.
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Thursday, April 26th 

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

Overview, Workshop Objectives, DNA Subway Tour

DNA Subway Workshop Introduction

 

10:15-10:30

iPlant Collaborative Overview

 Overview of iPlant Collaborative

www.iplantcollaborative.org

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to Teach Concepts in Molecular Biology

Red Line Lesson Sketch

What is a Gene? , Encode Paper

11:15-12:00

Setting up projects for Annotation - Red Line

 

 

12:00-01:00

Lunch

 

 

01:00-01:15

DNA Barcoding setup

 Plant DNA Barcoding

 

01:15-01:30

Mini-Seminar: DNA Barcoding

 

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 Gene Annotation Paper

03:15-04:45

Gene Annotation I

 

 

04:45-05:00

Mini-Seminar: Alternative Splicing                                                      

Alternative Splicing Intro

TMV and Alt Splicing , Plant Splicing , Jasmonate Splicing

Friday, April 27th

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              

 

Ping Paper , Target Paper, Epigenetic TE regulation in plants

09:30-10:15

Transposon Sleuthing: Yellow Line

 

 

10:15-10:30

Break

 

 

10:30-11:30

Gene Annotation II

 

 

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch

 

 

01:30-02:30

DNA Barcoding - Blue Line

Introduction to Blue Line                 

 

02:30-02:45

Break

 

 

02:45-03:45

Barcdoing electrophoresis and sequencing prep

 

 

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

http://www.learnaboutsma.org/   - SMA website and splicing animation.

http://www.iBoL.org - International Barcode of Life Project

DNA Subway exercises and handouts in word andpdf format. 

DNA Barcoding planning and prep section

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

https://www.surveymonkey.com/s/GenomicsInEducation_post

Post Workshop DNA Sequencing Results

Your DNA Sequencing results are now (5/2/2012) available on DNA Subway!

1.       Log into DNA Subway (dnasubway.iPlantcollaborative.org)

2.       Start a blue line project

3.       Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding

4.       Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number: 10-193907669 and are posted under Jermel Watkins on 5/2/12

5.       Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.

           For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....

Important Pre-Workshop Travel Info

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Check website like hotels.com and travelocity.com for additional hotels and rates. 

Hotel

Website

Notes

Motel 6

Motel 6 Website

~$50-$59 per night

Nearest Airports/ Rail

Airport

Distance

Notes

Louis Armstrong International Airport

~ 16 miles

http://www.flymsy.com/

Shuttle Services from Airports and Buses

Call ahead of time to reserve/confirm

Service

Notes

New Orleans Mass Transit

http://www.norta.com/

Ground Transportation from MSY

http://www.flymsy.com/PageDisplay.asp?p1=6016

Parking Information:

Free parking is available on the campus street adjacent to the New Science Building. Walk along Emmet Bashful Blvd. and Campus Blvd. to reach the New Science Building. Alternatively, you can drive in through Gentilly/Press Drive, and make a left onto Emmet Bashful Blvd. Immediately you will see a large Education Building to your right with an adjoining parking lot. Park in that lot and walk up Campus Blvd. on the right; the next building is the New Science Building. In the NSB take the elevator to the 3rd floor, Room 313, where you will receive a temporary Parking Permit to be placed on the dashboard of your car. This is to avoid receiving a parking violation from the campus police. 

On-Campus Directions:

See the campus map.