2012 03 27 Genomics in Education Workshop, Purdue University

2012 03 27 Genomics in Education Workshop, Purdue University

Organizers:

Jason Williams and Sheldon McKay

Local Point of Contact:

Burkhard Schulz

Trainers:

Jason Williams and Sheldon McKay

Date:

March 27-28

Location:

Purdue University Hall for Discovery and Learning Research (DLR), Science Lab DLRC 128,
207 South Martin Jischke Drive, Suite 203, West Lafayette , IN 47907-1791

Important Dates:

 

Section Navigation

Pre-Workshop Homework

Laptop Compatibility

If you are bringing your own laptop:

Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.

Here are some things to check:

  • If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
  • Ensure that your computer is running a current browser (Mozilla Firefox 3.6/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
  • If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install the newest version.
  • If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
  • To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot:JavaTest.pdf.

Make sure you have a valid iPlant account

Additional Readings and Assignments

  • Review the DNA Subway manual (a printed copy and some other handouts will be presented to you at the workshop).
  • Review the DNA Barcoding wet-lab.
  • Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
  • If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so! 
  • Check out the slides and papers mentioned in the agenda.

Workshop Agenda and Resources

Tuesday, March 27th

Time

Topic

Slides

Notes/Links

09:00-09:15

Welcome/Logistics

 

 

09:15-10:00

Introductions

 

 

10:00-10:15

Overview, Workshop Objectives, DNA Subway Tour

New DNA Subway workshop introduction1.ppt

 

10:15-10:30

iPlant Collaborative Overview

iPlant_intro.ppt

 

10:30-10:45

Break

 

 

10:45-11:15

Using DNA Subway to Teach Concepts in Molecular Biology

Red line lesson sketch.ppt

what is a gene.pdf,

11:15-12:00

Setting up projects for Annotation - Red Line

 

sequence_annotation_stein.pdf

12:00-01:00

Lunch

 

 

01:00-01:15

DNA Barcoding setup

 

 

01:15-01:30

Mini-Seminar: DNA Barcoding

plantdnabarcoding.ppt

 

01:30-02:45

DNA Barcoding wet-lab cont'd

 

 

02:45-03:00

Mini-Seminar: Evidence Based Gene Modeling

 

 

03:15-04:45

Gene Annotation I

 

http://gfx.dnalc.org/files/evidence/Annotation/

04:45-05:00

Mini-Seminar: Alternative Splicing

alternative splicing.ppt

alt splic TMV resistance.pdfgenome wide plant splicing.pdf

Wednesday, March 28th

Time

Topic

Slides

Notes/Links

09:00-09:30

Mini Seminar: Transposons                                                              


target_paper_2009.pdfping_naito_nature_2009.pdf

09:30-10:15

Transposon Sleuthing: Yellow Line

Green Revolution.ppt

                                                                                      

10:15-10:30

Break

                                                                 

 

10:30-11:30

Gene Annotation II

 

jasmonate and splicing.pdf

11:30-12:00

DNA Barcoding (PCR)

 

 

12:00-01:00

Lunch

 

 

01:30-02:30

DNA Barcoding - Blue Line

Blue_line_phylo_intro.ppt

Barcoding 101

02:30-02:45

Break

 

 

02:45-03:45

Barcdoing electrophoresis and sequencing prep

 

Genewiz, Promega Kit

03:45-04:30

Implementation and Classroom Planning

 

 

04:30-05:00

Summary, Evaluation, and Closure

 

 

Other Websites and Links and Resources

http://www.learnaboutsma.org/   - SMA website and splicing animation.

DNA Subway Handouts_word    - All the classroom handouts (with the exception of the chapter from "Genome Science") in word format

DNA Subway Handouts_pdf      - All the classroom handouts (with the exception of the chapter from "Genome Science") .pdf format

Teacher Planning for Barcoding - Additional information for doing the barcoding lab

Post Workshop Survey

Please complete this survey at the conclusion of the workshop:

https://www.surveymonkey.com/s/GenomicsInEducation_post

Post Workshop DNA Sequencing Results

Your DNA Sequencing results are now (4/2/2012) available on DNA Subway!

1.       Log into DNA Subway (dnasubway.iPlantcollaborative.org)

2.       Start a blue line project

3.       Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding

4.       Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number: 10-191487104 and are posted under Jermel Watkins on 3/31/12

5.       Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.

           For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....

Important Pre-Workshop Travel Info  

Recommended Lodging

There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.

Hotel

Website

Notes

Hilton Garden Inn

http://www.hiltongardeninn.com/en/gi/hotels/index.jhtml?ctyhocn=LAFWLGI

Close to campus

Holiday Inn

http://www.holidayinn.com/hotels/us/en/lafayette/lafin/hoteldetail                      

Downtown hotel

Union Club Hotel

http://www.union.purdue.edu/HTML/UnionClubHotel/

On campus/walking distance

Notes: Purdue provides a more extensive list of hotels here:http://www.homeofpurdue.com/stayingthenight.html.

Nearest Airports/ Rail

Airport

Distance

Notes

Indianapolis International

65 miles

 

Chicago O'Hare

145 miles

 

Chicago Midway

130 miles

 

Purdue University Airport

N/A

Private Planes Only Airport website may have useful travel info: http://www.purdue.edu/pat/

Amtrak

 

Amtrak stop at Lafayette w/connection to buses

Shuttle Services from Airports and Buses

Call ahead of time to reserve/confirm

Service

Route

Star of America*                             

Indianapolis International Airport to Purdue                                                                    

Lafayette Limo

Indianapolis International Airport to Purdue

Express Air Coach

Chicago O'Hare to Purdue

Greyhound

Transportation to Lafayette Station

CityBus

Lafayette bus system with stops at Purdue University

Parking Information:

Workshop participants may purchase a one-day A permit for $5 which allows you to park in the large Discovery Park Lot just across the street from the Discovery
Learning Center.Purdue staff who already have an A or B permit can also park in that lot.  Permits can be purchased at the Parking Facilities Office located in the
Visitor Information Center on Northwestern Ave.Office hours are 7:30 A.M. - 4:30 P.M., Monday – Friday.

On-Campus Directions:

Directions to Purdue University can be found at this link: http://admissions.purdue.edu/Visit_Programs/drivedirections.html.

Here is a campus map: http://www.purdue.edu/campus_map/Access2010-11.pdf