2012 03 27 Genomics in Education Workshop, Purdue University
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Local Point of Contact: |
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Trainers: |
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Date: |
March 27-28 |
Location: |
Purdue University Hall for Discovery and Learning Research (DLR), Science Lab DLRC 128, |
Important Dates: |
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Section Navigation
Pre-Workshop Homework
Laptop Compatibility
If you are bringing your own laptop:
Most laptops (<5 years old) updated with the latest web browser (we recommend FireFox:http://www.mozilla.org/en-US/firefox/fx/ ) will have no problem.
Here are some things to check:
- If possible, make sure you have have administrative privileges on your computer (should it become necessary to install or update software during the workshop).
- Ensure that your computer is running a current browser (Mozilla Firefox 3.6/ Internet Explorer 8 (IE 9 preferred) / Safari 4 or later).
- If you are a Windows user, ensure that your computer is running Java 6 Update 19 or later by going to http://java.com and click ‘Do I Have Java?’ Then click ‘Verify Java version’ and install the newest version.
- If you are a Mac user, check that your computer has the latest Java installed (probably Java 6) and that it also is enabled. (Check it out at http://java.com or contact williams@cshl.edu for additional advice).
- To test your Java installation, go to http://gfx.dnalc.org/files/apollo/test/ and follow the instructions at that site. The test is successful if an image opens up on your computer that looks like this screenshot:JavaTest.pdf.
Make sure you have a valid iPlant account
- You should be able to log into the DNA subway at: http://dnasubway.iplantcollaborative.org/.
- If you do not have an iPlant account, please get one at: http://user.iplantcollaborative.org/register.
Additional Readings and Assignments
- Review the DNA Subway manual (a printed copy and some other handouts will be presented to you at the workshop).
- Review the DNA Barcoding wet-lab.
- Bring a small sample of plant tissue (a leaf/flower/etc.) or collect one on campus before the experiment.
- If you wish to bring sequence data for the DNA barcoding or genome annotation experiments feel free to do so!
- Check out the slides and papers mentioned in the agenda.
Workshop Agenda and Resources
Tuesday, March 27th
Time |
Topic |
Slides |
Notes/Links |
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09:00-09:15 |
Welcome/Logistics |
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09:15-10:00 |
Introductions |
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10:00-10:15 |
Overview, Workshop Objectives, DNA Subway Tour |
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10:15-10:30 |
iPlant Collaborative Overview |
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10:30-10:45 |
Break |
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10:45-11:15 |
Using DNA Subway to Teach Concepts in Molecular Biology |
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11:15-12:00 |
Setting up projects for Annotation - Red Line |
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12:00-01:00 |
Lunch |
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01:00-01:15 |
DNA Barcoding setup |
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01:15-01:30 |
Mini-Seminar: DNA Barcoding |
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01:30-02:45 |
DNA Barcoding wet-lab cont'd |
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02:45-03:00 |
Mini-Seminar: Evidence Based Gene Modeling |
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03:15-04:45 |
Gene Annotation I |
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04:45-05:00 |
Mini-Seminar: Alternative Splicing |
alt splic TMV resistance.pdf, genome wide plant splicing.pdf |
Wednesday, March 28th
Time |
Topic |
Slides |
Notes/Links |
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09:00-09:30 |
Mini Seminar: Transposons |
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09:30-10:15 |
Transposon Sleuthing: Yellow Line |
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10:15-10:30 |
Break |
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10:30-11:30 |
Gene Annotation II |
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11:30-12:00 |
DNA Barcoding (PCR) |
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12:00-01:00 |
Lunch |
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01:30-02:30 |
DNA Barcoding - Blue Line |
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02:30-02:45 |
Break |
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02:45-03:45 |
Barcdoing electrophoresis and sequencing prep |
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03:45-04:30 |
Implementation and Classroom Planning |
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04:30-05:00 |
Summary, Evaluation, and Closure |
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Other Websites and Links and Resources
http://www.learnaboutsma.org/ - SMA website and splicing animation.
DNA Subway Handouts_word - All the classroom handouts (with the exception of the chapter from "Genome Science") in word format
DNA Subway Handouts_pdf - All the classroom handouts (with the exception of the chapter from "Genome Science") .pdf format
Teacher Planning for Barcoding - Additional information for doing the barcoding lab
Post Workshop Survey
Please complete this survey at the conclusion of the workshop:
https://www.surveymonkey.com/s/GenomicsInEducation_post
Post Workshop DNA Sequencing Results
Your DNA Sequencing results are now (4/2/2012) available on DNA Subway!
1. Log into DNA Subway (dnasubway.iPlantcollaborative.org)
2. Start a blue line project
3. Under 'Select Project Type' select the project type to be ‘rbcL’ barcoding
4. Under ‘Select Sequence Source’ choose ‘Import Sequence from DNALC’ Your sequences have a the tracking number: 10-191487104 and are posted under Jermel Watkins on 3/31/12
5. Select the sequences you want to include and run your project; each person should have two sequences, 1 forward, 1 reverse.
For example, Student 1 will have a sequence called Student1-M13F(21).... and a sample called Student1-M13R....
Important Pre-Workshop Travel Info
Recommended Lodging
There is no reimbursement for lodging. These hotels include those recommended by our hosting institution,but no guarantees are offered about the availability or suitability of the hotels.
Hotel |
Website |
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Hilton Garden Inn |
http://www.hiltongardeninn.com/en/gi/hotels/index.jhtml?ctyhocn=LAFWLGI |
Close to campus |
Holiday Inn |
http://www.holidayinn.com/hotels/us/en/lafayette/lafin/hoteldetail |
Downtown hotel |
Union Club Hotel |
On campus/walking distance |
Notes: Purdue provides a more extensive list of hotels here:http://www.homeofpurdue.com/stayingthenight.html.
Nearest Airports/ Rail
Airport |
Distance |
Notes |
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Indianapolis International |
65 miles |
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Chicago O'Hare |
145 miles |
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Chicago Midway |
130 miles |
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Purdue University Airport |
N/A |
Private Planes Only Airport website may have useful travel info: http://www.purdue.edu/pat/ |
Amtrak |
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Amtrak stop at Lafayette w/connection to buses |
Shuttle Services from Airports and Buses
Call ahead of time to reserve/confirm
Service |
Route |
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Indianapolis International Airport to Purdue |
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Indianapolis International Airport to Purdue |
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Chicago O'Hare to Purdue |
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Transportation to Lafayette Station |
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Lafayette bus system with stops at Purdue University |
Parking Information:
Workshop participants may purchase a one-day A permit for $5 which allows you to park in the large Discovery Park Lot just across the street from the Discovery
Learning Center.Purdue staff who already have an A or B permit can also park in that lot. Permits can be purchased at the Parking Facilities Office located in the
Visitor Information Center on Northwestern Ave.Office hours are 7:30 A.M. - 4:30 P.M., Monday – Friday.
On-Campus Directions:
Directions to Purdue University can be found at this link: http://admissions.purdue.edu/Visit_Programs/drivedirections.html.
Here is a campus map: http://www.purdue.edu/campus_map/Access2010-11.pdf