GOanna
Runs BLASTp of a single peptide FASTA file vs phylogenetic subsets of Uniprot accessions provided by AgBase. Transfers gene ontology (GO) annotations to from BLASTp matches to the query gene products.Combine GAFs
Combines gene association files (GAF) from InterProScan ( with XML parser) and GOanna.
Quick Start
- GOanna accepts a single peptide FASTA fileCombine GAFs accepts two GAF files.
- Resources: https://agbase.arizona.edu/cgi-bin/tools/GOanna.cgi, https://github.com/AgBase/GOannacombine_GAFs, https://agbase-docs.readthedocs.io/en/latest/
Test Data
| Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> GOannacombine_GAFs |
Input File(s)
Use CFLO_1.fa from _gaf.txt and clfo1_v_insecta_goanna_gaf.tsv from the directory above as test inputinputs.
Parameters Used in App
Use the Default parameters with the Test data.
Fill in the output section like so:
Output file basename: clfo1_vs_insecta
Database of query id: i5k
Assigned by field for your GAF output file: <your name>
Taxon ID of the peptide you are BLASTing: 29053the Output File Name filed with a suitable name for you output file.
Output File(s)
clfo1_v_insecta.asn–BLAST output for conversion to other formats
clfo1_v_insecta.html–pairwise alignments from BLAST search
clfo1_v_insecta.tsv--tab-delimited BLAST output
clfo1_v_insecta_goanna_gaf.tsv–gene association file with transferred GO annotationscomplete_CLFO_gaf.tsv is the combined GAF file