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Table of Contents

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De Novo Sequencing A process in which a novel genome is sequenced for the first time and requires specialized assembly of sequencing reads. For this tutorial the assembler Velvet will be used to assemble the genome.  A recommended approach will be followed in testing different kmer settings.

Velvet Velvet is a short read de novo assembler using de Bruijn graphs. More information about Velvet (including the manual) can be found here: http://www.ebi.ac.uk/~zerbino/velvet/

Workflow

This workflow is composed of two apps.

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  1. When run with the example data the workflow takes ~ 1 4 min to complete.
  2. All outputs from the workflow can be found in the 'Velveth' folder in the folder you specified for your outputs
  3. Your assembled contigs can be found in the file 'contigs.fa'.

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