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De Novo Sequencing A process in which a novel genome is sequenced for the first time and requires specialized assembly of sequencing reads. For this tutorial the assembler Velvet will be used to assemble the genome. A recommended approach will be followed in testing different kmer settings.
Velvet Velvet is a short read de novo assembler using de Bruijn graphs. More information about Velvet (including the manual) can be found here: http://www.ebi.ac.uk/~zerbino/velvet/
Workflow
This workflow is composed of two apps.
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- When run with the example data the workflow takes ~ 1 4 min to complete.
- All outputs from the workflow can be found in the 'Velveth' folder in the folder you specified for your outputs
- Your assembled contigs can be found in the file 'contigs.fa'.
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