Demultiplexes fastq files
Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> fastq_multx
Use the following files from the directory above as test input.
- Undetermined_S0_L001_I1_001.fastq use as index SEQFIL
- Undetermined_S0_L001_R1_001.fastq use as R1
- Undetermined_S0_L001_R2_001.fastq use as R2
Parameters Used in App
When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.
- Default parameters only, no further configuration needed.
Expect a fastq file for each barcode in the data set (2 for paired-end data) and a file for those where the barcode could not be determined.