Demultiplexes fastq files

Quick Start

Test Data

Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> fastq_multx

Input File(s)

Use the following files from the directory above as test input.

  • Undetermined_S0_L001_I1_001.fastq use as index SEQFIL
  • Undetermined_S0_L001_R1_001.fastq use as R1
  • Undetermined_S0_L001_R2_001.fastq use as R2

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.

  • Default parameters only, no further configuration needed.

Output File(s)

Expect a fastq file for each barcode in the data set (2 for paired-end data) and a file for those where the barcode could not be determined.

Tool Source for App