Fastq-multx-1.4.0
Demultiplexes fastq files
Quick Start
To use Fastq-multx-1.4.0, import your data fastq format.
Resources: https://github.com/ExpressionAnalysis/ea-utils/blob/wiki/FastqMultx.md
Test Data
Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> fastq_multx
Input File(s)
Use the following files from the directory above as test input.
Undetermined_S0_L001_I1_001.fastq use as index SEQFIL
Undetermined_S0_L001_R1_001.fastq use as R1
Undetermined_S0_L001_R2_001.fastq use as R2
Parameters Used in App
When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.
Default parameters only, no further configuration needed.
Output File(s)
Expect a fastq file for each barcode in the data set (2 for paired-end data) and a file for those where the barcode could not be determined.