Fastq-multx-1.4.0

Demultiplexes fastq files

Quick Start

To use Fastq-multx-1.4.0, import your data fastq format.
Resources: https://github.com/ExpressionAnalysis/ea-utils/blob/wiki/FastqMultx.md

Test Data

Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> fastq_multx

Input File(s)

Use the following files from the directory above as test input.

Undetermined_S0_L001_I1_001.fastq use as index SEQFIL
Undetermined_S0_L001_R1_001.fastq use as R1
Undetermined_S0_L001_R2_001.fastq use as R2

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.

Default parameters only, no further configuration needed.

Output File(s)

Expect a fastq file for each barcode in the data set (2 for paired-end data) and a file for those where the barcode could not be determined.

Tool Source for App

https://github.com/ExpressionAnalysis/ea-utils/blob/wiki/FastqMultx.md