HTSeq with BAM input, instead of SAM

Quick Start

App Creator

Roger Barthelson

Test Data

Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data ->htseq_with_BAM_input.

Input File(s)

Use hy5_rep1.bam and hy5_rep1_transcripts.gtf from the directory above as test input.

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the section below.


Use these parameters within the DE app interface:

    • MODE - union
    • feature type - exon
    • feature attribute - gene_id
    • default for the rest

Output File(s)

Expect a tab delimited file as output. For the test case, the output file you will find in the example_data directory is named FeatureCounts.

Related Tutorials

fRNAkenseq (HTseq-with-BAM-input) Tutorial

Tool Source for App