seqtk_seq

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Quick Start

• To use seqtk_seq, import your directory containing sequence data in Fasta/q format.
• *Resources: https://github.com/lh3/seqtk

Test Data

Input File(s)

• Use directory seqtk_seq_input from the directory above as directory containing sequence data.

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input directory to get the output provided in the next section below.

• Use these parameters within the DE app interface:
  • Select "64" in "quality shift"
  • Check "convert quality to Q33"

Advanced Use

• The sequence data should be in FASTA/Q format which can also be optionally compressed by gzip.
• You should select correct quality shift for you data. Default 33 is the most commonly used on current sequencing platforms.
• Check "convert quality to Q33" to convert fastq file with quality shift 33.
• You could mask bases with quality lower than a number you select and/or mask bases with quality higher than a number you select.
• You could select the character masked bases converted to, default to lowercase
• You could drop sequences with length shorter than a number you select and/or drop comments at the header lines

Output File(s)

Expect a directory named seqtk_seq_output as output directory containing converted sequence files.

Tool Source for App

• https://github.com/lh3/seqtk