Cuffmerge 2.2.1

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Cuffmerge merges together several Cufflinks assemblies. It also handles running Cuffcompare. The main purpose of this script is to make it easier to make an assembly GTF file suitable for use with Cuffdiff.

Quick Start

To use Cuffmerge, import 2 or more gtf files produced by Cufflinks
Resources:
- http://cole-trapnell-lab.github.io/cufflinks/cuffmerge/

Test Data

Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> cuffmerge

Input File(s)

Use

WT_rep1_transcripts.gtf

hy5_rep1_transcripts.gtf

from the directory above as test input.

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.

Default parameters only, no further configuration needed.

Output File(s)

Expect

merged.gtf and

merged_with_ref_ids.gtf

as output.

Tool Source for App

- http://cole-trapnell-lab.github.io/cufflinks/cuffmerge/