Poretools fasta 0.6.1a1

Extract FASTA sequences from a set of FAST5 files

Quick Start

• To use Poretools fasta 0.6.1a1, import your data in FAST5

• Resources: https://poretools.readthedocs.io/en/latest/

Test Data

Input File(s)

Use the following files from the directory above as test input:

• nanopore2_R9_MinKNOW_0.51_Ecoli_K12_MG1655_lambda_1004_1_ch106_read73_strand.fast5 
• nanopore2_R9_MinKNOW_0.51_Ecoli_K12_MG1655_lambda_1004_1_ch112_read42883_strand.fast5 
• nanopore2_R9_MinKNOW_0.51_Ecoli_K12_MG1655_lambda_2258_1_ch118_read1032_strand.fast5

Parameters Used in App

When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.

• Use these parameters within the DE app interface:
  • Set the output file name to 'fasta_output.fasta'
  • Leave all other parameters as default

Output File(s)

Expect fasta_output.fasta as output.

Tool Source for App

• https://poretools.readthedocs.io/en/latest/