Cuffmerge2
Cuffmerge2
Cuffmerge2 merges together several Cufflinks assemblies. It also handles running Cuffcompare. The main purpose of this script is to make it easier to make an assembly GTF file suitable for use with Cuffdiff.
Cuffmerge2 provides an interface to Cuffmerge 2+ to merge reference GTF files for multple Cufflinks outputs. This step is recommended by the authors of the Cufflinks package.
App Creator
Sheldon Mckay
Quick Start
- To use Cuffmerge2, import 2 or more gtf files produced by Cufflinks
- Resources: http://cole-trapnell-lab.github.io/cufflinks/cuffmerge/
Test Data
Input File(s)
Use
WT_rep1_transcripts.gtf
hy5_rep1_transcripts.gtf
from the directory above as test input.
Parameters Used in App
When the app is run in the Discovery Environment, use the following parameters with the above input file(s) to get the output provided in the next section below.
- Default parameters only, no further configuration needed.
Output File(s)
Expect
merged.gtf and
merged_with_ref_ids.gtf
as output.
Related Tutuorials
Tool Source for App
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