GACT
GACT: Genome Assembly Challenge Team
Objectives
The iPlant Collaborative is keenly interested in providing support for draft-level genome assembly via a combination of our resources and some of the machines available on the TeraGrid. The folks in this group are known to be doing assembly operations on a host of architectures, using a variety of algorithms and approaches, to assemble the genomes of a veritable garden of species. The group will discuss your current and future sequencing projects and will work to identify:
- Best practices and strategies for general sequencing bioinformatics
- Best practices and strategies for genome assembly
- An application stack that will be broadly useful on the various TeraGrid systems
- Updates to TACC's operations or system configurations that would facilitate genome assembly tasks
- The optimal systems on which to support genome assembly
- Potential ways to present basic assembly in the iPlant Discovery Environment
Plans and Progress
Deliverable |
Timeframe |
---|---|
Abyss PE assembler
|
progress:
|
FastQC tool for pre-filtering reads
|
progress:
|
Tools to support multiple, mixed size libraries for input into Abyss
|
progress:
|
Stand-alone Kmer analysis-Tallymer or custom script |
Fall 2011 (?) |
Basic statistics analysis on FASTA files
|
progress:
|
External advisers/users/testers
|
Ongoing |
Test RNA-seq transcriptome read data
|
progress:
|
Test Genomic data (model/non-model, sequence technologies) |
SRA? urgent |
Gene Space Discovery & Annotation
|
progress:
|
Additional Assembly tools
|
progress:
|
Read filters quality control
|
Progress:
|
Annotation Planning
- RNA assembly annotation
- DNA assembly annotation
Resources
- Mailing List: iPlant-GACT@iplantcollaborative.org
- Mailing List Archives: http://mail.iplantcollaborative.org/pipermail/iplant-gact/
Meeting Agendas and Notes
- GACT10: 10-13-2011
- GATC09: 8-03-2011
- GATC08: 7-28-2011
- GATC07: 7-19-2011
- GATC06: 7-14-2011
- GATC05: 7-06-2011
- GATC04: 6-28-2011
- GATC03: 6-20-2011
- GATC02: 6-15-2011
- Meeting #01 Feb XX, 2011
- https://pods.iplantcollaborative.org/wiki/display/sciplant/Genome+Assembly+Weekly+Updates* https://pods.iplantcollaborative.org/wiki/display/sciplant/Genome+Assembly+Weekly+Updates
Potential Outside Advisers
Outstanding Action Items
- None