Minimap2_index_align-2.10
Minimap2_index_align-2.10
This App runs Minimap2 (version 2.10) to:
find overlaps between long noisy reads, or map long reads or their assemblies to a reference genome.
App Creator
Amanda Cooksey
Quick Start
Minimap2 requires the following:
reference genome in fasta format (may be gzipped) or an index (.mmi) of the reference genome
long reads in fastq or fasta format (may be gzipped)
Resources
Test Data
Test data for this app appears directly in the Discovery Environment in the Data window under Community Data -> iplantcollaborative -> example_data -> minimap2
Input File(s)
Reference genome fasta file
GCF_000146045.2_R64_genomic.fna.gz
Oxford Nanopore reads file
SRR6059712.fastq
Parameters Used in App
In the Index Parameters section choose the reference genome fasta input file: 'GCF_000146045.2_R64_genomic.fna.gz'.
In the Index Parameters section enter a file name (index.mmi) in the 'Dump index to FILE' field to save the index file created by the app.
In the Mapping and Alignment section select the Oxford Nanopore reads input file: 'SRR6059712.fastq'.
In the Mapping and Alignment section select 'long-read spliced alignment' from the 'preset options' drop-down menu.
All remaining parameters may be left as default.
Output File(s)
index.mmi
This is the index file of the genome generated by the app. This file may be used in future analyses in place of the reference genome fasta file.
output
This is the alignment file generated by the app. It is in PAF format.